| Fragment Information | ||
|---|---|---|
| Fragment | Size (bp) | Conc. (ng/uL) |
| Fragment 1 | ||
| Fragment 2 | ||
| Fragment 3 | ||
| Fragment 4 | ||
| Overlapping PCR (100uL) | |
|---|---|
| Fragment 1 | |
| Fragment 2 | |
| Fragment 3 | |
| Fragment 4 | |
| 2x-pfu | 50.00 |
| N-sol | 8.00 |
| Primer For | 0.40 |
| Primer Rev | 0.40 |
| UPW | |
| Overlapping PCR (Library) | ||
|---|---|---|
| Library PCR Volume (uL): | ||
| Fragment 1 | ||
| Fragment 2 | ||
| Fragment 3 | ||
| Fragment 4 | ||
| 2x-pfu | ||
| N-sol | ||
| Primer F | ||
| Primer R | ||
| UPW | ||